Thermo Fisher, SR0234B, Oxoid™ ONE Broth-Listeria Supplement

Yield: Each vial for 2.25L medium
Unit Size: Each
Add to ONE Broth-Listeria, Part No. CM1066. ONE Broth - Listeria: Typical Formulation
• Peptone: 26g/L; Carbohydrate mix: 6g/L; Salt mix: 10g/L with a final pH of 7.4 ±0.2 at 25°C Appearance:
• Dehydrated medium: straw coloured, free-flowing powder
• Selective supplement: orange/yellow, freeze-dried pellet
• Prepared medium: dark straw coloured, clear liquid Note:Formulation Directions
• Suspend 22g of ONE Broth-Listeria Base in 500mL (44g/L) of distilled water
• Mix well and sterilize by autoclaving at 121°C for 15 minutes
• Cool the medium to below 50°C and aseptically add the contents of one vial of ONE Broth-Listeria Selective Supplement (Mfr. No. SR0234) reconstituted as directed Testing Protocol
• Add 25g of food sample to 225mL of ONE Broth-Listeria and stomach for a minimum of 30 seconds to mix the sample
• Incubate the broth without agitation at 30°C for 24±2 hoursGently agitate the bag then, using a microbiological loop, remove 10μL and inoculate onto a suitable agar medium e.g. Oxoid Brilliance Listeria Agar (ISO) (Mfr. No. CM1212, SR257 and SR0258) and incubate appropriately (37°C for 24-48 hours)
• Confirm presumptive colonies on the agar plates asListeria monocytogenesorListeriaspp. by appropriate methods e.g. Gram-stain, catalase, oxidase, Oxoid Listeria Latex Test Kit (Mfr. No. DR1126)
• Oxoid O.B.I.S. mono (Mfr. No. ID0600), Microbact Listeria 12L (Mfr. No. MB1128)