Description
No. of Wells: 20-well
Unit Size: 1 pack
The Novex Tris-Glycine Plus Midi Wedgewell Welcome Pack provides all the necessary Novex Tris-Glycine Plus Midi gels, buffers, and reagents you will need to begin using the SureLock Tandem Midi Gel Tank.Welcome Pack contents
• SureLock Tandem Midi Gel Tank (STM1001)
• Novex Tris-Glycine Plus WedgeWellMidi Gels (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer, 10X (LC2675)
• Novex Tris-Glycine SDS Sample Buffer, 2X (LC2676)
• NuPAGE Sample Reducing Agent, 10X (NP0004)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa (26619)About the SureLock Tandem Midi Gel TankThe SureLock Tandem Midi Gel Tank provides for rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a set-up time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. The tank is compatible with all Invitrogen pre-cast midi gels and midi gel cassettes.About Novex Tris-Glycine Plus Midi gelsNovex Tris-Glycine Plus midi gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus midi gels are a 1.0-mm-thick, wider-format (8 x 13 cm) gel for higher throughput electrophoresis of protein samples.Transfer and detectionFor transfer of proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer (LC3675) for traditional wet transfer using the SureLock Tandem Midi Blot Module (STM2001). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).Convenient to useNuPAGE Tris-Glycine WedgeWell-format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
