Thermo Fisher, T1012, Galacto-<i>Star</i>™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells, 600 Assays

Name: Galacto- Star ™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells, 600 Assays
Brand: Thermo Fisher
SKU: T1012

Quantity: 600 Assays, Unit Size: 600 assays

Catalog Number: T1012

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Category

Western Blot Detection Reagents

Pack Size600 assays

TagsProtein Analysis Reagents Gene Reporter Systems

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 600 Assays
Unit Size: 600 assays
• Glow assay kinetics provides awindowduring which measurements may be performed, facilitating HTS applications where assay automation is used
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
• Assay can be completed in about one hour, providing fast assay turnaroundSensitiveThe chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.VersatileThe Galacto-Star system includes Galacton-Star chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.Long-Lasting SignalLight emission from the Galacto-Star assay reaches maximum in 60 to 90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.Specifications
• Assay Type: β-Gal (lacZ) Assay
• Compatible Cells: Mammalian Cells
• Detection Method: Chemiluminescent
• For Use With Equipment: Luminometer (Microplate)
• Form: Liquid
• Format: 96-well plate
• High Throughput Compatibility: High Throughput-Compatible
• Readout: End Point
• Sample Type (Specific): Cell Cultures
• Substrate: Galacton-Star
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase

Specifications

General

Assay	β-Gal (lacZ) Assay
Compatible Cells	Mammalian Cells
Detection Method	Chemiluminescence
For Use With (Application)	Enzyme Assay
For Use With (Equipment)	Luminometer (Microplate)
Form	Liquid
High-throughput Compatibility	High-throughput Compatible
Label or Dye	Unconjugated
Product Line	Galacto-Star, NovaBright
Product Type	β-Galactosidase Reporter Gene Assay System
Quantity	600 Assays
Readout	End Point
Sample Type	Cell Cultures
Substrate	Galacton-Star™
Substrate Properties	Chemical Substrate
Substrate Type	Beta-Gal Substrate
Target Enzyme	Beta-Galactosidase
Unit Size	600 assays

Ordering

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