Description
No. of Wells: 12-well
Unit Size: 1 kit
The NuPAGE Tris-Acetate WedgeWell Welcome Pack provides all the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the XCell SureLock Mini-Cell.Welcome Pack contents
• XCellSureLock Mini-Cell(EI0001)
• NuPAGE Tris-Acetate mini WedgeWell gels (2 boxes, 20 gels)
• NuPAGE Tris-Acetate SDS Running Buffer, 20X (LA0041)
• NuPAGE LDS Sample Buffer, 4X (NP0007)
• NuPAGE Sample Reducing Agent, 10X (NP0009)
• HiMark Pre-stained Protein Standard (LC5699)About the XCell SureLock Mini-Cell The XCell SureLock Mini-Cell is a vertical mini-protein gel electrophoresis system compatible with all Invitrogen mini precast gels and SureCast handcast gels. The Mini-Cell is simple, sturdy, and easy to use. Each XCell SureLock Mini-Cell can accommodate up to two gels per run. Run times may vary depending on buffer conditions and power supplies used.About NuPAGE Tris-Acetate gelsNuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional Laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer (NP0006) for traditional wet transfer using the XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).Convenient to useNuPAGE Tris-Acetate WedgeWell-format gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
