Description
Fill Weight: 10 ± 1mL
Manufacturing Origin: Germany
Unit Size: Each
Muller-Kauffmann Tetrathionate-Novobiocin Broth was developed by Muller^2 and later modified by Kauffmann^3,4 with the addition of ox bile and brilliant green to improve selectivity. The addition of novobiocin at 40 mg per liter was later described by Jeffries^5 to improve inhibition of Proteus species.After the sample has been incubated in Buffered Peptone Water (ISO), transfer 1 mL of the broth to 10 mL of MkTTn Broth and 0.1 mL into 10 mL of RVS Broth (TV5036E). Incubate the MkTTn Broth at 37°C ± 1°C for 24 hours ± 3 hours and the RVS Broth at 41.5°C ± 1°C, for 24 hours ± 3 hours. Sub-culture the incubated MkTTn and RVS broths onto XLD Agar (PO5057A/PO0164A) and a second agar medium of choice, and incubate for a further 24 hours ± 3 hours at 37°C ± 1°C. Presumptive Salmonella should be confirmed using appropriate biochemical and serological techniques. For the complete method, please refer to ISO 6579:2002^1.Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand, combining powerful manual, semi-automated and fully automated test products and a comprehensive line of media and diagnostic products to offer a complete, end-to-end solution to quickly deliver the products you need and the quality results your laboratory depends on.
