Thermo Fisher, V53220, pBudCE4.1 Mammalian Expression Vector

Quantity: 20 μg
Unit Size: 20 µg
• Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in cell; this can eliminate variable expression due to differences in gene copy number
• Provides expression cassettes with the CMV promoter for high-level transcription of genes with optionalc-mycepitope tag for rapid detection and 6xHis sequence for simple purification
• Human EF-1α promoter for high-level expression of genes with optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
• Sh ble(ZeoR) gene for efficient selection in both mammalian cells andE. coliwith selection agent Zeocin™Constitutive Expression, Mammalian Expression, Multi-Gene Expression, Protein Expression, Proteins, Expression, Isolation and Analysis