| Chemistry | C8 |
|---|---|
| Separation Mode | Reversed Phase |
| Particle Substrate | Silica |
| pH Range Min | 2 pH |
| pH Range Max | 8 pH |
| Maximum Pressure | 6000 psi (415 Bar) |
| Endcapped | Yes |
| Silanol Activity | Low |
| Particle Shape | Spherical |
| Particle Size | 3.5 µm |
| Endfitting Type | Waters |
| Pore Size | 100 Å |
| Format | Column |
| Surface Area | 335 |
| System | HPLC |
| USP Classification | L7 |
| Inner Diameter | 4.6 mm |
| Length | 150 mm |
| Carbon Load | 12 % |
| UNSPSC | 41115709 |
| Brand | Symmetry |
| Product Type | Columns |
| Units per Package | 1 pk |
Answer:It uses endcapped octyl-bonded silica, a reversed-phase chemistry with different retention from conventional C18.
Answer:The column measures 4.6 × 150 mm and contains 3.5 µm particles with 100 Å pores.
Answer:The stated pH range is 2–8, and the maximum operating pressure is 415 bar.
The Waters Symmetry C8 HPLC column provides reversed-phase separation with an octyl-bonded stationary phase for compounds requiring less hydrophobic retention than a typical C18 material. This analytical format uses 3.5 µm high-purity silica particles with 100 Å pores in a 4.6 × 150 mm column. The base-deactivated silica and endcapped C8 chemistry are intended to limit secondary interactions, supporting reproducible peak shapes. Because C8 and C18 phases have different ligand lengths and retention characteristics, changing to this column may alter elution order, resolution and run time. The specified pH range is 2 to 8 and the maximum column pressure is 415 bar. Establish suitable solvent ratios, flow rates and injection concentrations during method transfer. Consistent column equilibration, clean solvents and filtered samples help maintain performance over successive runs. For contamination-prone samples, a matching guard column or inline filter may reduce fouling. The product contains one complete analytical HPLC column, not a guard insert or chromatography resin bottle.
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