DL30S05-3008WT, YMC-Pack Diol
column
Catalog Number: DL30S05-3008WT
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Category
TagsDihydroxypropyl
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| Stationary Phase | YMC-Pack Diol (dihydroxypropyl) |
|---|---|
| Pore Size | 300 Å |
| Particle Size | 5 µm |
| Column Length | 300 mm |
| Column ID | 8.0 mm |
Answer:Size-exclusion chromatography with hydrophilic diol-bonded silica.
Answer:300 mm long and 8.0 mm internal diameter.
Answer:300 Å (30 nm) pores and 5 µm particles.
The YMC-Pack Diol-300 column performs size-exclusion chromatography for macromolecules using a hydrophilic diol-bonded silica packing. The Diol-300 phase features 300 Å pores and 5 µm particles in a 300 × 8.0 mm analytical column, combining substantial pore accessibility with a long separation bed. Size-exclusion chromatography separates molecules primarily by hydrodynamic size: larger species access less of the porous volume and generally elute ahead of smaller species. The 30 nm pore class is relevant to protein separations and molecular-size characterization within the method's supported range. Compatible aqueous buffered mobile phases can reduce unwanted secondary interactions with the stationary phase. Select mobile-phase composition, injection volume, protein concentration and flow rate to limit aggregate formation and avoid overloading the bed. Calibration with appropriate molecular-size standards is required when estimating apparent molecular weight from retention. Protect the column from particulates and sample precipitation. This product is a prepacked SEC analytical column, not a diol HILIC column or bulk resin.
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