BD
10X Zinc Fixative (Formalin Free)
SKU · 552658
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Flow cytometry lives or dies on signal quality and reproducibility. This Collection brings together BD Biosciences reagents—antibodies, viability dyes, buffers, compensation beads, and fix/perm solutions—so you can design cleaner multicolor panels, reduce staining artifacts, and move from experiment plan to gated results with fewer surprises. As a distributor, Iright offers a wide selection of BD flow reagents with reliable sourcing, invoicing, and technical support.
Before diving into product families, it helps to clarify what you gain: consistent staining across complex panels, options to balance brightness against antigen abundance, and utilities that tame compensation headaches. With Iright, you can source the right BD reagent mix, get SKU-level accuracy, and keep your panel design workflow simple.
BD’s portfolio aims at real panel pain points: higher-brightness fluorochromes for rare targets, buffers that mitigate staining artifacts when multiple Brilliant dyes are used, and standardized kits for intracellular staining. These elements shorten the path from panel idea to interpretable gates.
A clear map helps you navigate fast. Use this overview to locate the reagent type you need, then jump to selection tips and example use cases. Each family supports a specific stage of your staining workflow.
Now that you’ve seen the lineup, let’s connect products to outcomes. This section focuses on practical gains—resolution, stability, and panel scalability—so your design decisions are driven by effect, not guesswork.
Brighter signals and clearer separation. BD Horizon Brilliant fluorochromes provide high brightness across common lasers, improving rare-population resolution and enabling cleaner bivariate gating. Pair high-brightness colors with low-abundance antigens to lift weak signals out of the background.
Fewer staining artifacts in complex panels. When using multiple Brilliant dyes, Brilliant Stain Buffer and BSB Plus help mitigate artifacts and preserve expected expression patterns. BSB Plus keeps the mitigation benefits while supporting reduced staining volumes, which is useful when the sample or reagent is limited.
Reliable intracellular workflows. Cytofix/Cytoperm provides a standardized fix/perm process and includes Perm/Wash for consistent intracellular staining results without sacrificing your surface staining strategy.
Reproducible compensation and unmixing. CompBeads and SpectraComp particles deliver consistent positive/negative controls to set compensation matrices or spectral unmixing, reducing run-to-run variability.
Flexible color choices. OptiBuild on-demand reagents open additional dye–clone combinations so you can add markers or shift colors to cleaner channels as your panel evolves.
Good panels match color brightness to antigen biology and instrument reality. Use this quick guide as a checklist when you assemble or expand a panel—especially beyond 8–10 colors.
Examples help you translate principles into carts. Treat these as modular patterns you can adapt to your markers and instruments.
As you refine your panel, you’ll encounter these names frequently. Understanding what each line does helps you select intentionally and avoid trial-and-error.
This table summarizes where each category fits in your workflow. Use it to spot gaps, avoid redundant steps, and pair reagents effectively.
| Category | Typical Use | Key Advantages | Pair With | Notes / Representative SKUs |
|---|---|---|---|---|
| Antibodies (Horizon/Brilliant/Pharmingen) | Surface or intracellular (with fix/perm) | Wide clone & color coverage; high brightness for rare targets | BSB/BSB Plus; viability dye; CompBeads | Choose brightness by antigen abundance |
| Viability Dyes | Live/dead discrimination | Cleaner gates; fewer false positives | Any panel | Add early; keep timing consistent |
| Brilliant Stain Buffer (BSB) | Panels using ≥2 Brilliant dyes | Mitigates staining artifacts in complex panels | Brilliant-conjugated antibodies | 563794 (100 tests), 566349 (1000 tests) |
| Brilliant Stain Buffer Plus | Low-volume staining with Brilliant dyes | Artifact mitigation at reduced volumes | Brilliant-conjugated antibodies | 568264 (100 tests) |
| Cytofix/Cytoperm Kit | Intracellular cytokines/transcription factors | Standardized fix/perm; preserves surface staining strategy | Intracellular antibody cocktails | 554714 kit; includes 554722, 554723 |
| Compensation / Spectral Beads | Compensation matrix / unmixing controls | Reproducible positive/negative populations | Any multicolor panel | 552843 (CompBeads Mouse), 560497 (CompBead Plus Mouse) |
Many panel issues repeat across labs. These answers focus on practical, searchable questions so you can troubleshoot quickly and choose with confidence.
What’s the difference between BSB and BSB Plus?
Both mitigate staining artifacts when multiple Brilliant dyes are used; BSB Plus is formulated for reduced staining volumes without sacrificing mitigation. Choose BSB for standard volumes and BSB Plus when sample or reagent is limited.
How do I set up intracellular staining properly?
Stain surface markers first, then use the Cytofix/Cytoperm Kit to fix and permeabilize, followed by intracellular antibodies in Perm/Wash buffer. Keep timing and temperatures consistent to protect epitope integrity.
Which beads should I use for compensation or spectral unmixing?
Use BD CompBeads to generate consistent positive/negative populations for traditional compensation. For spectral systems, SpectraComp™ particles provide unmixing controls across multiple species. Always run single-color controls that match your fluorochromes.
Do I need a viability dye in every panel?
If your sample prep risks dead cells (most do), a viability dye is strongly recommended. It reduces background, improves gating accuracy, and stabilizes population frequencies across replicates.
Whether you are expanding to a 14-color panel or standardizing an intracellular protocol, we can help you choose the right antibodies, buffers, BSB/BSB Plus, fix/perm kits, and compensation beads—then deliver them reliably. Share your cytometer/laser setup, target list, and test volume; we’ll suggest suitable dye–clone options (including OptiBuild when helpful), confirm BD SKUs, and align lead times with your schedule.
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