Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 7 pg/mL,
Range:8.23 - 6000 pg/mL,
Assay Platform:Microplate
Product details:
Abcam's CCL23 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL23 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL23 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CCL23 ELISA Kit (ab216169) from our SimpleStep ELISA
range.
Optimisation may be required with urine samples.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCL23 also known as macrophage inflammatory protein 3 is a chemokine involved in immune response regulation. It has a molecular weight of approximately 15-16 kDa and is secreted by various cells such as macrophages dendritic cells and endothelial cells. CCL23 is expressed mostly in the bone marrow and lymph nodes but it also localizes in tissues like the liver and lungs under certain conditions. It plays an important role in mediating chemotaxis directing the migration of cells toward infection sites.
Biological function summary
CCL23 functions in the recruitment of immune cells especially eosinophils and monocytes to areas where immune responses are necessary. This chemokine is not known to be part of a larger protein complex but its ability to interact with specific receptors on cell surfaces makes it integral to immune signaling. During immune activation CCL23 contributes to the inflammatory cascade influencing both acute and chronic inflammatory responses.
Pathways
The signaling functions of CCL23 integrate within the chemokine signaling pathway and the broader immune response pathway. CCL23 interacts closely with proteins such as CCR1 which acts as its receptor and influences cellular responses via this receptor-mediated signaling. Additionally CCL23 shares mechanistic parallels with other chemokines like CCL22 though they may attract different subsets of cells they participate in coordinated immune activities.
CCL23 has relevance to conditions such as rheumatoid arthritis and allergic reactions. In these diseases elevated levels of CCL23 and its interaction with other proteins such as CCR1 contribute to the recruitment and activation of inflammatory cells. This chemokine's role in immune cell trafficking and inflammation makes it a target for therapeutic interventions aimed at modulating immune responses in such disorders.
