Description
Key facts
Sample types:Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 0.04 µM,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
The detection limit is 2 pmol per assay (or 40 nM concentration) of H2O2 with the more sensitive fluorometric readout.
The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.
Hydrogen peroxide assay principle
In the hydrogen peroxide assay protocol, horse radish peroxidase (HRP) reacts with a probe and H2O2 to produce a product with color (λmax = 570 nm) and red-fluorescence (Ex/Em=535/587 nm).
Hydrogen peroxide assay protocol summary:
- Add standards and samples to wells
- Add reaction mix and incubate for 10 min
- Analyze with microplate reader
How other researchers are using
Hydrogen Peroxide Assay Kit has been used in a variety of sample type including:
- Mouse breast tumor cells
- Mouse embryonic fibroblasts
- Vaginal strains
References:
1- Zhou N et al. 2023; 2- Venit T et al. 2023
3- Takano T et al. 2023
Related and recommended products
See other alternative kits to quantify Hydrogen Peroxide:
- Hydrogen Peroxide Assay Kit (Cell-based)
ab138874
- Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared)
ab138886
Other notes
This product was previously called K265 Biovision Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
