Description
Key facts
Sample types:Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 2.5 µM,
Range:2.5 - 250 µM,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Coenzyme A Assay Kit ab102504 provides an easy, accurate assay to measure the CoA level in biological samples. The assay can detect 0.1 to 10 nmol of CoA (2.5-250 uM concentration range) in a variety of samples.
Coenzyme A assay principle
In the Coenzyme A assay, Acyl-CoA synthetase acts on Coenzyme A to produce Acyl-CoA. The action of Acyl-CoA oxidase on Acyl-CoA then produces hydrogen peroxide, which reacts with the OxiRed Probe. This produces a colored (570 nm) and fluorescent (Ex/Em 535/587 nm) readout.
Coenzyme A assay protocol summary
- add samples and standard to wells
- add ACS Substrate and CoA Converter Mix and incubate for 30-min at 37°C
- add reaction mix and incubate for 30-min at 37°C
- analyze with a plate reader
Other Notes
This product was previously called K367 Biovision Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Coenzyme A (CoA) is an important enzyme cofactor with a molecular mass of approximately 767.5 Da. Alternate names include CoA-SH and acetyl coenzyme A when bonded to an acetyl group. CoA is widely expressed in both prokaryotic and eukaryotic cells including the liver heart and brain tissues playing a significant role in metabolic processes. It functions mechanically by carrying acyl groups such as acetate which is vital for the conversion of pyruvate in the Krebs cycle and fatty acid synthesis.
Biological function summary
This cofactor is important for various enzymatic reactions especially those involving the metabolism of carbohydrates fats and amino acids. It is not a part of a larger protein complex but works closely with enzymes like acetyl-CoA carboxylase. Its ability to form energy-rich thioester bonds allows for the transfer of acyl groups making it essential for energy production and biosynthetic reactions in cells. CoA transfers these acetyl groups in forms like acetyl coenzyme A or acetyl CoA which are critical for cellular functions.
Pathways
CoA is essential in both the citric acid cycle (Krebs cycle) and fatty acid metabolism. In the citric acid cycle it helps convert pyruvate into acetyl-CoA therefore linking glycolysis to energy production. In fatty acid metabolism CoA participates in the process of beta-oxidation. Both pathways involve enzymes such as pyruvate dehydrogenase and fatty acid synthase which collaborate with CoA in important steps.
Perturbations in CoA metabolism are associated with conditions such as neurodegenerative diseases and metabolic disorders like diabetes. Alterations in CoA levels may affect pathways involving proteins such as pyruvate dehydrogenase and acetyl-CoA carboxylase which are important in maintaining cellular energy balance and lipid metabolism. These links highlight the importance of CoA in health and disease contexts.
