Description
Key facts
Sample types:Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 0.1 mM,
Range:0.2 - 1 mM,
Assay time:20m,
Assay Platform:Microplate reader
Product details:
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.2 – 1 mM).
How the assay works
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
Calcium assay protocol summary
- Add samples and standards to wells
- Add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- Analyze with microplate reader
Related Calcium assay products
Calcium assay kit ab102505 is designed for use with liquid samples such as biological fluids, cell lysates, and tissue extracts.
For the analysis of calcium flux in cell cultures in imaging assays, we supply Fluo-8 Calcium Flux Assay Kits. Calcium Flux Assay Kit (Fluo-8, No Wash)
ab112129
is generally preferred as the workflow is simpler. The alternative is Medium removal kit Fluo-8 Calcium Flux Assay Kit
ab112128
. Note - calcium flus assays are also referred to as calcium influx assays.
For the analysis of calcium flux in cultured cells in a flow cytometer, we supply Calcium Flux Assay Kit (Flow cytometry) (ab233472)
For the imaging of calcium deposits in paraffin sections, we supply Von Kossa (Calcium Stain) Kit
ab150687
The Calcium Assay Buffer component is also available under
ab182458
Getting the best performance from ab102505
This calcium assay detects free calcium ions only.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calcium often referred to as Ca2+ acts mechanically as an important signaling molecule and structural element in biological systems. It is a small ion with an atomic mass of 40.08 Daltons which allows it to easily move across membranes. You can find calcium expressed in various tissues prominently within bones teeth and intracellular compartments such as the endoplasmic reticulum and mitochondria. As an ion calcium plays an integral role in cellular signaling processes enabling numerous functions including muscle contraction and neurotransmitter release.
Biological function summary
Calcium modulates various cellular activities serving as a messenger in signal transduction pathways. It participates in forming complexes with proteins like calmodulin and troponin which subsequently activate an array of cellular processes. Calcium is also pivotal in maintaining the structural integrity of bones through its deposition as hydroxyapatite. Calcium ions influence cellular excitability and are involved in numerous physiological processes through their interaction with various ion channels enzymes and proteins.
Pathways
Calcium plays critical roles in several including the phosphoinositide signaling pathway and the muscle contraction pathway. In these pathways calcium influx and release modulate activities of proteins like calmodulin and phospholipase C. Calcium’s interactions facilitate the propagation of intracellular signals impacting cell proliferation differentiation and programmed cell death. These pathways highlight calcium’s role in maintaining cellular homeostasis and function.
Calcium imbalance influences conditions such as osteoporosis and cardiac arrhythmias. Dysregulation of calcium homeostasis affects bone density leading to osteoporosis a disorder characterized by increased bone fragility. Furthermore abnormal calcium handling in cardiac myocytes contributes to arrhythmias as calcium ions regulate cardiac muscle excitability and contractility. Proteins like calmodulin and troponin linked with calcium play roles in these conditions highlighting the clinical impact of calcium signaling disturbances.
