Description
Key facts
Sample types:Urine, Plasma, Cell culture media, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:< 10 µM,
Assay time:30m,
Assay Platform:Microplate reader
Product details:
Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.
How the assay works
This ammonia assay using a modified Berthelot reagent is based on a reaction between ammonia and an alkaline reagent that produces a blue-green colored product. The intensity of the color is proportional to the amount of ammonia in the sample. The colored product is indophenol, which is easily quantifiable by colorimetry (λ=670nm) using a plate reader.
Berthelot ammonia assay protocol summary
- Add samples and standards to wells
- Add reagent 1 and reagent 2 and incubate for 30 min at at 37°C
- Analyze with a microplate reader
How other researchers are using ab102509
Ammonia Assay Kit - Modified Berthelot (Colorimetric) has been used with a variety of sample types including:
Escherichia coli BL21(DE3) cells
Ureaplasma cells
Human iPS(Foreskin)
References: 1-Srinivasachari S et al. 2023, 2-Fleming D et al. 2022, 3-Pettinato G et al. 2021
Related and recommended products
Ammonia assay kit
ab83360
is an alternative to this product, and is the most popular ammonia assay kit.
Other notes
This product was previously called K470 Biovision Ammonia Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ammonia a small molecule with a molecular mass of about 17 daltons is an important player in nitrogen metabolism. It exists primarily as ammonium (NH4+) in physiological pH environments. Ammonia is found predominantly in liver cells where it is produced through amino acid deamination and is further involved in urea production. Ammonia detection can be performed using various tools such as the ammonia detection kit ammonia test kit and the ammonia measurement reader which are essential for researchers to analyze and quantify its presence.
Biological function summary
Ammonia participates in the nitrogen cycle being a substrate for urea synthesis in the liver a vital part of nitrogen waste removal. It combines with carbon dioxide to form carbamoyl phosphate which enters the urea cycle a complex enzymatic system within mitochondria and cytosol. Ammonia's role is not isolated; it interacts with enzymes such as glutamine synthetase. The ammonium assay and ammonia assay provide insights into ammonia's role and concentration helping elucidate its biological functions and impact.
Pathways
Ammonia is important in the urea cycle and gluconeogenesis. In the urea cycle it is converted to urea preventing toxic accumulation. Gluconeogenesis involves ammonia as it aids in converting pyruvate to glucose a fundamental process for energy maintenance. Related proteins in these pathways include carbamoyl phosphate synthetase in the urea cycle and pyruvate carboxylase in gluconeogenesis. A colorimetric assay might be used to evaluate ammonia's integration in these pathways.
Ammonia plays a significant role in hepatic encephalopathy and hyperammonemia both linked with improper ammonia metabolism. Excess ammonia in the blood due to liver dysfunction can lead to hepatic encephalopathy affecting brain function. Glutamine synthetase activity correlates to ammonia levels impacting the severity of these conditions. Ammonia assay kits are fundamental tools in assessing the association of ammonia with these disorders facilitating better understanding and management of the conditions.
