Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Extracts, Cell culture media, Cell culture extracts, Milk, Serum,
Assay type:Enzyme activity,
Sensitivity:= 0.02 mU/well,
Assay time:1h 30m,
Assay Platform:Microplate reader
Product details:
How the assay works
Lipase assay kit measure the activity of lipase in a sample by using a reaction that produces a color or fluorescence. The amount of the color or fluorescence produced is proportional to the amount of lipase in the sample. In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).
This lipase assay kit detects lipase activity as low as 0.02mU per well.
Lipase Assay Kit (Colorimetric)summary:
- Add samples and standards to wells
- Prepare standard curve
- Add reaction mix
- Measure optical density (OD570 nm) in a kinetic mode for 60-90 min at 37°C on a microplate reader
How other researchers are using
Lipase Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse colon and pancreas samples
Mouse lung cancer samples
Blood samples from mice with pancreatitis
References:
1-Xia H et al. 2023
2-Bartolacci C et al. 2022
3-Tarasiuk A et al. 2021
Related and recommended products
See other alternative kits to quantify lipase activity :
- Lipase Assay Kit III (Fluorometric)
ab118969
- Lipase Assay Kit II (Colorimetric)
ab102525
This product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
