Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Urine (UTI), Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Enzyme activity (quantitative),
Sensitivity:> 1 mU/mL,
Range:1 - 100 mU/mL,
Assay time:30m,
Assay Platform:Microplate reader
Product details:
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.
In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.
How the LDH assay works
In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.
LDH assay protocol summary
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C
Getting the best performance from ab102526
LDH activity is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.
Related LDH assay kits
To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit
ab65393
, which is designed specifically as a cytotoxicity assay.
This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit
ab197000
Other notes
This product was previously called K726 Biovision Lactate Dehydrogenase Activity Colorimetric Assay Kit. Abcam acquired Biovision in 2021.
How other researchers have used LDH Assay Kit ab102526
This LDH assay kit has been used in publications in a variety of sample types, including:
- Human: serum
- Mouse: kidney tissue
, kidney epithelial cell line lysates
, muscle tissue
, serum
, vaginal lavage fluid
, liver tissue
- Rat: H8C2 cell lysates
It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures
, THP-1 cells
, primary chondrocyte cells on a membrane scaffold
, primary lymphocytes
, ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay
References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.
Biological function summary
Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.
Pathways
LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.
Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.
