Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture extracts, Tissue Extracts, Serum,
Assay type:Quantitative,
Sensitivity:> 0.5 µg,
Range:0.5 - 30 µg,
Assay time:1h 30m
Product details:
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
How the assay works
The BCA assay method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample.
The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml). Support on how to determine protein concentration can be found
Getting the best performance
The BCA assay protocol is very flexible. Both the incubation time and temperature can be varied over a rather wide range. Lower protein samples can be more easily quantified using higher temperatures and longer incubation times.
When assaying protein in solutions containing detergent, best results are obtained by adding the same amount of detergent to the wells containing the protein standard.
BCA assay protocol summary
- mix Copper Reagent and BCA Reagent to make BCA assay working solution
- dispense samples and standards into 96-well plate
- add working solution to each well
- shake gently and incubate for 30-90 minutes
- measure absorbance in a plate reader at 562 nm
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols
