Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum, Urine,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:= 2.44 ng/mL,
Range:10 - 500 ng/mL,
Assay Platform:Microplate
Product details:
Cortisol ELISA kit ab108665 is a competitive ELISA kit to measure total human cortisol in serum and plasma with a sensitivity of 2.44 ng/mL.
How the assay works
Cortisol ELISA kit ab108665 is a competitive ELISA. There are 2 types of competitive ELISAs:
antigen down
: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
antibody down
: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
