Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Adenovirus IgM Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Adenovirus in Human serum and plasma.
A 96-well plate has been precoated with Adenovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Adenovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adenovirus IgM sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adenovirus IgM is an antibody with a molecular weight of approximately 900 kDa. It is mainly expressed in the blood during the acute phase of adenovirus infections. Known for its pentameric structure Adenovirus IgM plays an important role in early immune response by targeting adenovirus capsid proteins. It forms part of the larger immunoglobulin family and marks the initial phase of serological detection following viral exposure.
Biological function summary
This immunoglobulin marks recent adenovirus infection and initial immune activation. During infections the immune system produces Adenovirus IgM which binds to the viral antigen hindering viral replication and promoting viral clearance. Whilst not part of large protein complexes it interacts directly with adenovirus structural proteins. This interaction helps in activating the complement system and facilitating phagocytosis by immune cells.
Pathways
The immune response against adenovirus involves the classical complement pathway and antibody-mediated antigen recognition. Adenovirus IgM initiates the complement cascade leading to opsonization and lysis of virus particles. It shares its function with Adenovirus IgG as both counter the viral infection. However IgM functions earlier than IgG during the infection timeline. The antibody's activity is important for the swift clearance of the virus.
Adenovirus can cause acute respiratory diseases and conjunctivitis. Adenovirus IgM serves as an indicator to diagnose these conditions in their early stages. Its presence signals the body’s initial immune response to these infections. The detection of IgM also connects with elevated levels of other acute phase proteins like C-reactive protein (CRP) which serve as markers of inflammation during infection.
