Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Ascaris lumbricoides IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Ascaris lumbricoides in Human serum and plasma.
A 96-well plate has been precoated with Ascaris lumbricoides antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Ascaris lumbricoides-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ascaris lumbricoides IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ascaris lumbricoides IgG also known as anti-Ascaris IgG plays an important role in immune response against Ascaris lumbricoides infections. This IgG antibody targets antigens presented by the parasitic roundworm Ascaris lumbricoides a significant human pathogen. The molecular mass of specific IgG antibodies can vary due to their structural diversity but they typically range around 150 kDa. Expression of this immunoglobulin occurs in the serum of individuals exposed to Ascaris infection as the immune system produces it to recognize and neutralize the roundworm.
Biological function summary
Anti-Ascaris IgG is important in the adaptive immune response. It helps identify and opsonize Ascaris lumbricoides facilitating their phagocytosis by immune cells like macrophages. This IgG is not part of a larger complex; instead it functions independently to combat the pathogen. The production of Ascaris IgG indicates exposure and an active immune response to infection which contributes to preventing invasion and damage by the parasite.
Pathways
Anti-Ascaris IgG is integral to immunological pathways involving antigen recognition and clearance. It is part of the humoral immune response pathway where it interacts with antigens of Ascaris lumbricoides. This antigen–antibody binding activates the complement pathway enhancing the ability of immune cells to clear pathogens. Additionally Ascaris IgG is associated with other immune proteins like complement protein C3 which further aids in the opsonization and destruction of the parasitic cells.
Anti-Ascaris IgG plays a role in the detection and management of ascariasis an infection caused by the Ascaris lumbricoides parasite. The presence of Ascaris IgG in serum serves as a marker for diagnosing this parasitic infection. Ascariasis can lead to complications including malnutrition and intestinal blockage. In relation to inflammation chronic Ascaris infections can stimulate IgE production contributing to allergic responses and potentially exacerbating asthma. Therefore alongside IgE Ascaris IgG assists in diagnosing and understanding the disease progression in affected individuals.
