Description
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Borrelia burgdorferi IgM Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).
A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Borrelia burgdorferi IgM sometimes referred to as B. burgdorferi IgM is an immunoglobulin M (IgM) antibody that specifically targets the outer surface proteins of the Borrelia burgdorferi bacterium. This bacterium is the primary causative agent of Lyme disease. Mechanically these antibodies are detectable using techniques like the Borrelia test kit or Lyme ELISA which identify the presence of Borrelia burgdorferi IgM in the blood. The detection of IgM antibodies indicates an early immune response and suggests that the body recently encountered the pathogen. These antibodies are typically expressed in the serum of infected individuals.
Biological function summary
The identification of Borrelia burgdorferi IgM involves the host’s immune response against the bacterial pathogen responsible for Lyme disease. These IgM antibodies play an important role in the initial immune response by binding to antigens present on the Borrelia bacterium. This binding initiates immune reactions like complement activation which aids in the elimination of the pathogen. Borrelia-specific IgM antibodies are not part of a larger protein complex but rather indicate the early phase of humoral immunity.
Pathways
Borrelia burgdorferi IgM involvement connects to key immune pathways such as the complement pathway and acute phase response. Detection of these antibodies signals the activation of the host's immune system indicating an ongoing or recent immune defense against the infection. While not directly interacting with specific proteins the presence of Borrelia burgdorferi IgM suggests interactions with components of these immune pathways which include other immune effectors and molecules.
Borrelia burgdorferi IgM is significantly associated with Lyme disease an infectious condition transmitted through the bite of infected Ixodes ticks. The detection of these IgM antibodies can help in diagnosing Lyme disease during its early stages. Furthermore the persistence of IgM alongside IgG antibodies can indicate chronic infection or re-infection playing a role in post-treatment Lyme disease symptoms. The interaction of Borrelia burgdorferi IgM with the immune system can also lead to complications in diagnosing other spirochetal infections due to potential cross-reactivity with antigens from similar pathogens.
