Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Human Anti-Candida albicans IgG ELISA Kit ab108715 is an indirect ELISA kit for the qualitative measurement of Human Anti-Candida albicans IgG in Citrate plasma, Heparin Plasma, Serum.
How the assay works
A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.
Assay protocol summary
Human Anti-Candida albicans IgG ELISA Kit ab108715 protocol summary:
1. Add samples and controls to wells used.Incubate at 37°C.
2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature.
3. After washing, add TMB substrate solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.
Getting the best performance
ASSAY SPECIFICITY
The specificity is > 95 % and is defined as the probability of the assay scoring negative in the absence of the specific analyte.
ASSAY SENSITIVITY
The sensitivity is 93 % and is defined as the probability of the assay scoring positive in the presence of the specific analyte.
INTERFERENCES
Interferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg/mL hemoglobin, 5 mg/mL triglycerides and 0.2 mg/mL bilirubin.
Related and recommended products
Human Anti-Candida albicans IgG ELISA Kit ab108715 is commonly used to study candida albicans and infections.
Other ELISA kits for measuring immune responses to Candida albicans are:
- Human Anti-Candida albicans IgA ELISA kit
ab108714
- Human Anti-Candida albicans IgM ELISA kit
ab108716
-Human Calprotectin ELISA kit (S100A8/S100A9)
ab267628
Human Anti-Candida albicans IgG ELISA Kit ab108715 is an indirect ELISA kit for the qualitative measurement of Human Anti-Candida albicans IgG in Citrate plasma, Heparin Plasma, Serum .
How the assay works
A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.
Assay specificity
The specificity is > 95 % and is defined as the probability of the assay scoring negative in the absence of the specific analyte.
The sensitivity is 93 % and is defined as the probability of the assay scoring positive in the presence of the specific analyte.
Interferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg/mL hemoglobin, 5 mg/mL triglycerides and 0.2 mg/mL bilirubin.
Human Anti-Candida albicans IgG ELISA Kit ab108715 protocol summary
1. Add samples and controls to wells used. Incubate at 37єC
2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature
3. After washing, add TMB substrate solution to each well. Incubate at room temperature
4. Add Stop Solution to each well. Read immediately
Related and recommended products
Human Anti-Candida albicans IgG ELISA Kit ab108715 is commonly used to study candida albicans and infections.
Other ELISA kits for measuring immune responses to Candida albicans are:
- Human Anti-Candida albicans IgA ELISA kit
ab108714
- Human Anti-Candida albicans IgM ELISA kit
ab108716
- Human Calprotectin ELISA kit (S100A8/S100A9)
ab267628
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
