Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Dengue virus ELISA kit - IgM is designed for the accurate qualitative measurement of IgM class antibodies against Dengue virus in Human serum and plasma.
A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgM sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dengue Virus IgM also known as anti-Dengue IgM is an antibody target involved in the immune response against the Dengue virus. This immunoglobulin M (IgM) is produced soon after Dengue virus infection. While the exact mass of Dengue Virus IgM is not specific to a single value typical IgM has a mass of approximately 950 kDa. Dengue Virus IgM can be detected in human serum or plasma indicating an acute phase of infection. It's not a protein but an antibody produced by B cells when the immune system encounters the Dengue virus.
Biological function summary
Anti-Dengue IgM antibodies play a role in the early immune response to Dengue infection. They form part of the humoral immune system and contribute to pathogen neutralization. These antibodies can exist as pentameric complexes that enhance their ability to bind to the virus. Once produced they help prevent the virus from entering host cells and assist other components of the immune system in identifying and eliminating the virus. Their presence indicates an active or recent Dengue virus infection making them important in Dengue serology.
Pathways
Dengue Virus IgM is part of the immune pathway responding to viral infection. Such pathways include the complement activation pathway where IgM antibodies activate the complement system to eliminate pathogens efficiently. Additionally they can interact with Fc receptors on immune cells enhancing phagocytosis of the virus. Through these interactions Dengue Virus IgM aids in the broader adaptive immune response involving helper T cells and other immunoglobulins such as IgG.
Dengue Virus IgM is directly associated with Dengue fever an acute viral illness spread by mosquitoes. The presence of Dengue Virus IgM in a host is a clinical indicator of Dengue virus infection and plays an important role in diagnostic assessments using methods such as IgM capture ELISA or Dengue ELISA kits. This antibody can contribute to immune-mediated reactions such as immune enhancement where the presence of different non-neutralizing antibodies may facilitate severe illness. IgM aids in differentiating between primary and secondary Dengue infections which differ in severity and immune response potentially influencing the risk of developing more serious conditions such as Dengue hemorrhagic fever.
