Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Human Anti-Influenza virus A IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Influenza virus A in human plasma and serum samples.
How the assay works:
A 96-well plate has been precoated with Influenza virus A antigens to ind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgG sample captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Anti-Influenza virus A IgG ELISA Kit ab108745 protocol summary
1. Add samples and controls to wells used. Incubate at 37°C
2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature
3. After washing, add TMB substrate solution to each well. Incubate at room temperature
4. Add Stop Solution to each well. Read immediately.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Influenza virus A IgG is an immunoglobulin G antibody that targets influenza virus A. Immunoglobulins are critical components of the immune system often used as diagnostic markers. The mass of IgG varies due to its complex structure generally around 150 kDa. This antibody is primarily expressed by B lymphocytes in response to influenza virus infection and plays a central role in identifying and neutralizing virus particles. The utilization of IgG in applications like Influenza ELISA test and Influenza assay kits highlights its importance in laboratory settings.
Biological function summary
The influenza virus IgG antibody binds specifically to antigens present on the influenza virus A tagging them for destruction by other immune cells. It does not typically form part of larger protein complexes but operates in concert with other components of the immune system like macrophages and natural killer cells to neutralize the virus. The presence and quantity of influenza IgG antibodies are pivotal indicators of past exposure or vaccination against flu virus A in ELISA testing.
Pathways
This antibody fits into the immune system pathway where it plays a role in activating the classical complement pathway. This pathway leads to opsonization of the virus therefore improving phagocytosis efficacy by macrophages. Additionally influenza virus A IgG interacts with Fc receptors on immune effector cells facilitating processes such as antibody-dependent cellular cytotoxicity (ADCC). These interactions highlight the relationship between influenza IgG and other immune receptors in mounting an effective response.
The influenza virus A IgG is closely related to influenza a respiratory illness caused by the influenza virus A. Monitoring IgG levels can help assess immunity status and guide vaccination strategies. Furthermore disturbances in IgG production might correlate with immune-related disorders such as immunodeficiencies or allergies. The presence of IgG is pivotal for the diagnosis and management of influenza and sometimes connects to adaptive immune responses involving proteins like the T-cell receptor during immune system adaptations.
