Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Serum,
Reacts with:Rabbit,
Assay type:Competitive,
Sensitivity:>= 0.15 µg/mL,
Range:0.195 - 200 µg/mL,
Assay time:3h,
Assay Platform:Microplate
Product details:
Albumin Rabbit ELISA Kit ab108793 is a competitive ELISA kit to measure Rabbit Albumin in Serum, Plasma, and Urine with a sensitivity of 0.15 µg/mL.
How the assay works
Rabbit Albumin ELISA Kit ab108793 employs an Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with wash buffer. Strepatvidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Strepatvidin-Peroxidase enzymatic reaction. TMB is catalyzed by Strepatvidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
Assay Specificity
This kit recognizes both native rabbit albumin in serum, plasma, and urine samples.
Albumin Rabbit ELISA Kit ab108793 protocol summary:
1. Add standard or sample to each well used and add prepared biotin protein to each well. Incubate at room temperature.
2. Wash each well and add Streptavidin Conjugate Solution. Incubate at room temperature.
3. Add TMB Solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.
How other researchers are using Albumin Rabbit ELISA Kit ab108793
Albumin Rabbit ELISA Kit ab108793 has been used to study the contribution of renal nerves in rabbit models for chronic kidney disease.
References:
Y. Sata, et.al., 2020, PMID: 32895021
Get results in 90 minutes with our SimpleStep ELISA
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
