Description
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.22 ng/mL,
Range:0.391 - 25 ng/mL,
Assay time:4h,
Assay Platform:Microplate
Product details:
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 is a sanwich ELISA to measure Human alpha 1 Antitrypsin (SERPIN A1) in Cerebral Spinal Fluid (CSF), Milk, Plasma, Saliva, Serum, Urine with a sensitivity of 0.26 ng/mL.
How the assay works
An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.
Assay specificity
The standard has been calibrated against WHO International Standard.
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 protocol summary
1. Add standards or samples to the wells, incubate 2 hrs
2. Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
3. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
4. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
5. Add 50 µl of Stop Solution per well. Read at 450 nm immediately
How other researchers are using Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 has been used in publications to develop liver organoids from human PSCs
, urinary biomarker panels for endometriosis
, and mRNA therapies for alpha 1-antitrypsin deficiency
References:
Gareth J Sullivan et al. 2023 PMID: 37653039,
Ting-Chang Chang et al. 2022 PMID: 35453583,
Ewa Ellis et al. 2020 PMID: 32341402
Get results in 90 minutes our SimpleStep ELISA® kit:
- Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)
ab189579
Related and recommended products
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab108799 is commonly used to study liver function and inflammation.
Other kits for measuring liver function and inflammation include:
- Human Albumin ELISA kit
ab179887
- Human ALT ELISA kit
ab234578
- Human AST ELISA Kit
ab263881
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.
Biological function summary
A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.
Pathways
Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.
Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.
