Description
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 4 ng/mL,
Range:0.008 - 0.5 µg/mL,
Assay time:4h,
Assay Platform:Microplate
Product details:
Human Apolipoprotein E ELISA Kit (APOE) ab108813 is a sandwich ELISA kit to measure Human Apolipoprotein E in Serum, Plasma, and Cerebral Spinal Fluid with a sensitivity of 4 ng/mL.
How the assay works
Human Apolipoprotein E ELISA Kit (APOE) ab108813 uses a pair of antibodies each specific of different epitopes at the target of interest : capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay Specificity
This kit recognizes Apo E2, Apo E3, and Apo E4 isoforms. This assay recognizes both natural and recombinant human Apo E. No significant cross-reactivity observed with Apo A1, Apo A2, Apo A4, Apo A5, Apo B, Apo C1, Apo C2, Apo C3, Apo H, Apo J, and Apo M.
Human Apolipoprotein E ELISA Kit (APOE) ab108813 protocol summary
1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature
3. Wash each well and add Streptavidin Solution. Incubate at room temperature
4. Add TMB Solution to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately
How other researchers are using Human Apolipoprotein E ELISA Kit (APOE)] ab108813
Human Apolipoprotein E ELISA Kit (APOE) ab108813 has been used to study lipid metabolism
, the role of oxidative stress in gestational diabetes
, and identify diagnostic biomarkers for tuberculous meningitis in CSF
.References:
G. Reyes-Soffer et al., 2017, PMID: 27986651,br>
M. Li et al., 2022, PMID: 34929050,br>
M. Huang et al., 2022, PMID: 36003300
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
Biological function summary
ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.
Pathways
In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.
In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.
