Description
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.16 ng/mL,
Range:0.313 - 20 ng/mL,
Assay time:4h,
Assay Platform:Microplate
Product details:
Abcam’s alpha 1 Microglobulin Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Microglobulin concentrations in Human plasma, serum, milk, urine, saliva, and CSF samples.
An alpha 1 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 Microglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-1-microglobulin also known as alpha-1-microglycoprotein or A1M is a protein with a mass of approximately 26 kDa. It is mainly expressed in the liver and secreted into the blood plasma. Alpha-1-microglobulin can bind to small peptides and free radicals acting as a scavenger. It exists in a monomeric form and is present in various bodily fluids including urine and amniotic fluid.
Biological function summary
This protein serves as a protective factor against oxidative stress. Alpha-1-microglobulin is part of the lipocalin protein family which has roles in transport of small hydrophobic molecules in extracellular spaces. Its antioxidant properties help protect biological macromolecules from damage induced by oxidative agents. Alpha-1-microglobulin contains heme-binding sites which contribute to its protective biochemical actions.
Pathways
Alpha-1-microglobulin participates in oxidative stress-related and inflammatory pathways. Within the oxidative stress pathway it interacts with heme oxygenase and other heme-binding proteins to mitigate toxic effects of excess free heme. In inflammatory pathways it links with proteins such as albumin and immunoglobulin components which further supports immune response and cellular protection mechanisms.
Alpha-1-microglobulin has associations with conditions like preeclampsia and kidney disease. In preeclampsia its levels can indicate oxidative stress and placental dysfunction involving interactions with other proteins such as haptoglobin. In kidney disease altered levels of alpha-1-microglobulin in urine serve as a biomarker for tubular dysfunction connecting it to conditions affecting renal function and health.
