Description
Key facts
Applications:Flow Cyt, Fluorescence Microscopy, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Target:DRAQ7See target data,
Form:LiquidSee storage information,
Storage buffer:Constituents: 1% Proprietary derivative of 1,5-BIS{[2-(DIMETHYLAMINO) ETHYL]AMINO}-4,8-DIHYDROXYANTHRACENE-9,10-DIONE
Product details:
DRAQ7™ is a far-red fluorescent dye that
stains the nuclei in
and permeabilized cells and can be used in combination with common labels such as GFP or FITC.
DRAQ7 is the ideal tool to study dead or membrane-compromised cells because it does not enter intact, live cells. It is an ideal replacement for propidium iodide and 7-AAD, as is not excited by UV light and has no emission overlap with PE / PE homologues.
Key features of DRAQ7 include:
Rapid staining of dsDNA/ nuclei of dead or permeabilized cells
Low photobleaching
It can be used in most cell types, eukaryotic and prokaryotic: mammalian, bacterial, parasitic, plant, etc ...
Non-toxic in long-term culture
Can be combined with "live" dyes
No compensation needed with common FITC/GFP + PE combinations in flow cytometry
No wash or RNase treatment needed.
SPECTRAL PROPERTIES
Excitation:
633 and 647 nm line optimal (Ex
599 / 644 nm)
488, 514 and 568 nm lines, sub-optimal (only by flow cytometry)
Emission (instrument dependent):
665 nm to infra-red max 678 nm / 697 nm intercalated with dsDNA)
Minimal overlap with vis range e.g. GFP and FITC
Em. filters may include 695L, 715LP or 780 LP
Multi-wavelength imaging with UV / vis fluorochromes
No fluorescence enhancement upon DNA binding
Low photo-bleaching effect
Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluoroscence microscopes
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze, Store in the dark
