Description
Key facts
Reacts with:Mouse, Rat, Cow, Human,
Assay type:Quantitative
Product details:
250 μg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 μg of Complex III from heart mitochondria.
ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857)
/MS910).
Related products
Review the
mitochondrial assay guide
, or the full
metabolism assay guide
to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complex III also known as cytochrome c reductase or cytochrome bc1 complex plays an important role in the mitochondrial electron transport chain. This multiprotein complex weighing approximately 250 kDa facilitates electron transfer from ubiquinol to cytochrome c. Complex III resides in the inner mitochondrial membrane of eukaryotic cells and is important for cellular energy production. Structurally it consists of 11 subunits in mammals with cytochrome b cytochrome c1 and Rieske iron-sulfur protein being the core components.
Biological function summary
Complex III transfers electrons and contributes to proton translocation across the mitochondrial membrane. This process generates the proton gradient needed for ATP synthesis via oxidative phosphorylation often referred to as OXPHOS. As a core component of the mitochondrial respiratory chain Complex III interacts closely with other complexes mainly Complexes I and IV to maintain efficient energy conversion.
Pathways
Complex III is integral to the oxidative phosphorylation pathway and the respiratory electron transport chain. It ensures efficient electron transport working closely with Complexes I II and IV. Cytochrome c acts as an electron carrier between Complex III and Complex IV in this pathway. This coordinated interaction is essential for ATP generation meeting cellular energy demands.
Complex III is linked with mitochondrial disorders and neurodegenerative diseases. Deficiencies or mutations in its components may lead to disorders such as mitochondrial encephalopathy or Leigh syndrome which are associated with impaired energy metabolism. The complex's dysfunction also affects proteins connected to these diseases like ATP synthase due to disrupted energy production. Additionally its role in oxidative stress has implications for conditions like mitotox-related mitochondrial damage.
