Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Purified mitochondria, Cell Lysate,
Reacts with:Mouse, Rat, Cow, Human,
Assay type:Enzyme activity,
Assay time:3h,
Assay Platform:Microplate reader
Product details:
Complex II Activity Assay Kit (Colorimetric) ab109908 is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.
Succinate, Ubiquinone 2, DCPIP and Phospholipids should be stored at -80°C. All other components should be stored at 4°C.
This product was previously called Complex II Enzyme Activity Microplate Assay Kit
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.
Biological function summary
Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.
Pathways
Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).
Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.
