Description
Key facts
Detection method:Colorimetric,
Reacts with:Human, Rat, Cow,
Assay type:Enzyme activity,
Assay Platform:Microplate reader
Product details:
ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Fumarase sometimes referred to as fumarate hydratase is an enzyme involved in the Krebs cycle. Its mechanical function involves the reversible hydration/dehydration of fumarate to malate. The enzyme has a molecular mass around 50 kilodaltons. Fumarase expression occurs in the mitochondria of eukaryotic cells and it also appears in the cytosol contributing to the compartmentalization of its enzymatic activity.
Biological function summary
Fumarase plays a critical role in cellular respiration by facilitating the conversion of fumarate to malate within the tricarboxylic acid (TCA) cycle. This enzyme does not operate as part of a larger protein complex but its activity is essential for maintaining the flow of carbon through the TCA cycle which is integral to energy production. Its activity is upregulated when cells require increased ATP generation.
Pathways
Fumarase participates prominently in the TCA cycle a vital pathway for aerobic respiration where it interconverts fumarate and malate. The enzyme supports cellular energy production and this is closely linked with other TCA cycle enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase which further facilitate efficient energy conversion.
Fumarase deficiency leads to severe neurological impairment often manifesting as encephalopathy. This deficiency occurs due to mutations in the FH gene which encodes for fumarase. Additionally a reduction in fumarase activity associates with fumarase-related hereditary leiomyomatosis and renal cell cancer (HLRCC) linking fumarase with altered metabolic states in tumorigenesis. Dysregulation within this context often involves interactions with metabolically similar enzymes complicating the metabolic landscape and contributing to oncogenic processes.
