Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Formate Assay Kit ab111748 is a simple and direct colorimetric assay for detecting formate in biological samples.
Formate assay principle
In the formate assay protocol, formate is oxidized to generate a product resulting in color formation (450 nm) proportional to formate concentration. The amount of formate in the sample is then determined by comparing the color to a standard curve.
Formate assay protocol summary
- Add samples, standards and positive control to wells
- Add formate reagent
- Incubate for 60 minutes at at 37°C.
- analyse with plate reader.
Other Notes
This product was previously called K653 Biovision Formate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Formate a simple anion derived from formic acid plays a significant role in metabolic processes. Known by its systematic name methanoate formate is a small molecule with a molar mass of 46.03 g/mol. It is found across various tissues where it participates in one-carbon metabolism. Cells generate formate through the breakdown of certain amino acids a pathway present in both mitochondria and cytosol. These sites of expression indicate the key involvement of formate in cellular energy dynamics.
Biological function summary
Formate acts as an important intermediary in one-carbon units transfer. It participates in the folate cycle where it serves as a donor of one-carbon units in the synthesis of purines and thymidine. Formate does not work alone; it often functions as part of a complex with other molecules involved in one-carbon metabolism pathways. The transfer of one-carbon units is essential for nucleotide biosynthesis and has implications in cellular proliferation and repair processes.
Pathways
Formate takes part actively in the folate and methionine cycles. These pathways play roles in cellular growth and methylation processes. Related proteins include methylenetetrahydrofolate dehydrogenase which facilitates formate's conversion into other active metabolic forms. Formate's role connects with pivotal cellular pathways that maintain homeostasis and growth implicating its significance in the broader metabolic network.
Formate's metabolism links to conditions such as folate deficiency and cancer. Deficient folate levels may lead to impaired nucleotide synthesis with consequences for DNA repair mechanisms while altered formate metabolism is observed in certain cancers hinting at increased proliferation requirements. Proteins like dihydrofolate reductase which interacts with folate metabolism relate formate's biological activity to these disease states. Understanding the roles of formate and its associated proteins can offer insights into potential therapeutic targets.
