Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Recommended alternative kit
We recommend that you purchase Resazurin Assay Kit
ab129732
instead of this kit.
Resazurin Assay Kit ab112119 (Cell Cytotoxicity, Fluorometric) ab112119 provides a fast, simple, accurate and homogeneous assay for the fluorometric detection of viable cells.
The resazurin assay protocol is based on the reduction of oxidized non-fluorescent blue resazurin to a red fluorescent dye (resorufin) by the mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. Resorufin has Ex/Em of 530-560 / 590 nm.
The resazurin assay is very commonly used to quantify the numbers of live cells, and monitor cell viability / cytotoxicity.
ab112119 is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are stable and have minimal cytotoxicity, thus a longer incubation time (such as 24-48 hours) is possible if required.
Resazurin assay protocol summary:
- add assay solution to cells in 96 or 384 well plates and shake gently for 30 sec
- incubate for 1-24 hr at 37°C
- analyze with microplate reader
ab112119 should be stored desiccated
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
