Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Direct,
Assay Platform:Flow cytometer
Product details:
Abcam's Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). ab112130 uses TF2-VAD-FMK as a fluorescent indicator for most caspase activities. The cell permeable and nontoxic TF2-VAD-FMK irreversibly binds to activated caspase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. Within 15 minutes incubation, it starts to react with active caspase enzymes.ab112130 provides all the essential components with an optimized assay protocol. It is designed to detect cell apoptosis by measuring generic activation of caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) in live cells.ab112130 is used for either the quantification of most activated caspase activities in apoptotic cells or screening of caspase inhibitors. TF2-VAD-FMK, the green label reagent, allows for direct detection of activated caspases in apoptotic cells by a flow cytometer at Ex/Em = 488/520 nm. Visit our for tips and troubleshooting.
ab112130 should be stored dessicated.**Other caspase and apoptosis assays**Review the full set of , or the .
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
