Description
Key facts
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Fluorescence microscope
Product details:
Lysosomal Staining Kit - Blue Fluorescence | Cytopainter (ab112135) is designed to label lysosomes in live cells in blue fluorescence at Ex/Em = 350/440nm. ab112135 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time.
ab112135 can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112135 provides all the essential components with an optimized cell-labeling protocol and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).
Review other dyes and kits for
lysosomal staining
, or the
live cell staining fluorescent dyes guide
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria,and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
