Description
Key facts
Detection method:Fluorescent,
Sample types:Cell culture extracts, Tissue Extracts, Cell culture supernatant,
Assay type:Semi-quantitative,
Sensitivity:= 40 ng/well,
Assay time:30m,
Assay Platform:Microplate reader
Product details:
Lysyl Oxidase Activity Assay Kit / LOX Activity Assay Kit (Fluorometric) (ab112139) provides a simple method to measure lysyl oxidase (LOX) activity in cell and tissue extracts from mammals and other species, as well as physiological solutions.
The LOX activity assay protocol uses a proprietary LOX substrate that releases hydrogen peroxide upon transformation by the LOX present in the sample. Hydrogen peroxide is in turn detected using a red fluorescence substrate for HRP-coupled reactions. This leads to increase in fluorescence that can be easily detected at Ex/Em 540/590 nm in a fluorescence microplate reader.
This assay is semi-quantitative as it does not contain a LOX standard for calibration. When a known concentration of LOX is used, the assay can detect activity from as low as 40 ng of lysyl oxidase in solution.
The assay is highly sensitive and its unique detection method eliminates the interference that occurs in certain biological samples.
Lysyl oxidase (protein-lysine-6-oxidase, LOX, EC 1.4.3.13) is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors.
The activity of Lysyl oxidase in biological samples is traditionally assessed by tritium release end-point assays using radio isotope labeled collagen or elastin substrates.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
