Description
Key facts
Detection method:Fluorescent,
Assay type:Quantitative,
Assay Platform:Microplate reader
Product details:
ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 uL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to
ab112140
, this fluorometric assay is more sensitive, and has less interference from biological samples.
Please note
this kit is optimized for the detection of small molecule-based maleimide.
If your sample is maleimide conjugated in protein, then this kit may not be suitable.
ab112141 should be stored desiccated.
This product was previously called Maleimide Quantification Assay Kit (Fluorometric)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Maleimide also called MBS is not a protein itself but an important synthetic chemical compound commonly used in bioconjugation. Maleimide has a molecular weight of 97.09 g/mol. Scientists employ maleimide in various applications mostly within laboratory settings rather than it being naturally expressed in biological tissues. Its reactivity with thiol groups makes it an effective tool for linking molecules such as in antibody-drug conjugates.
Biological function summary
Maleimide-thiol reactions facilitate covalent linkage to thiol-containing biomolecules allowing the creation of conjugates and functionalized surfaces. Maleimide beads often functionalized with maleimide groups are used to immobilize and purify proteins. Using maleimide assay kits researchers can measure the activity and kinetics of thiol-maleimide reactions. While not a natural biological entity maleimide integrates into biomolecular complexes by bridging different biomolecules for studies.
Pathways
Protein modification by maleimide supports research in signal transduction and metabolism. For example maleimide linkage methods help explore the interactions within the MAPK signaling pathway and the ubiquitin-proteasome system. By facilitating the study of these pathways maleimide assists in dissecting protein functions and interactions with related proteins like MAP kinases and ubiquitin ligases.
Research uses maleimide to investigate cancer and neurodegenerative diseases. Its role in attaching functional tags or drugs to proteins helps in cancer therapeutics especially in targeting specific oncogenes. Additionally maleimide's utility in studying protein aggregation links it to neurodegenerative diseases such as Alzheimer's where it helps dissect the roles of proteins like tau and amyloid-beta.
