Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Multi drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP dependent drug efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug resistance associated protein (MRP1) are members of the ABC transporter family. Abcam's Fluorimetric MDR Assay Kits use a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporters, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Abcam's Fluorimetric MDR Assay Kits are ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MDR also known as Multidrug Resistance protein 1 or P-glycoprotein (P-gp) is an ATP-dependent efflux pump with a molecular weight of approximately 170 kDa. This protein is integral to the plasma membrane and is expressed in various tissues including the liver kidney intestine and blood-brain barrier. MDR1 serves as a critical part of the body's defense mechanism actively exporting a wide range of substrates including drugs and toxins out of cells thereby influencing drug absorption and disposition.
Biological function summary
MDR1 plays a major role in protecting tissues by limiting drug penetration and facilitating the excretion of xenobiotics. It is part of the ATP-binding cassette (ABC) transporter family which forms a complex infrastructure of proteins responsible for the transport of molecules across cellular membranes. Its ability to pump out chemotherapy drugs significantly impacts treatment efficacy especially in certain cancer types where overexpression can lead to multidrug resistance.
Pathways
MDR1 interacts closely within the drug metabolism and transport pathways. It plays a vital role in the pharmacokinetic properties of drugs affecting both their distribution and elimination processes. MDR1 works in concert with other proteins in the ABC transporter family like MRP1 and BCRP which also contribute to drug resistance and excretion. The balance and activity of these transporters directly influence therapeutic outcomes and toxicity of cancer chemotherapeutic agents.
MDR1 is well recognized for its association with cancer particularly in its role in developing resistance to chemotherapy. Its overexpression is also noted in disorders involving drug resistance such as epilepsy where it affects the brain's protective barriers potentially limiting antiepileptic drug access. Together with proteins like LRP and BCRP MDR1 contributes to the complexity of drug delivery and efficacy in these conditions challenging clinical treatments and necessitating specific MDR assay kits for effective monitoring and management.
