Description
Key facts
Detection method:Fluorescent,
Sample types:Purified protein, Tissue Lysate,
Assay type:Direct,
Assay Platform:Microplate reader
Product details:
MMP Activity Assay Kit (Fluorometric Red) ab112147 uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of one part is quenched by the other. Upon cleavage into two separate fragments by MMPs, the fluorescence is recovered.
The MMP assy is designed to check the general activity of a MMP enzyme in a tissue sample. It can also be used to screen MMP inhibitors when a purified MMP enzyme is used.
With excellent fluorescence quantum yield and longer wavelength, the substrate used in this assay shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate.
The MMP assay signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The pH-independent fluorescence makes the assay reading available for the whole physiological pH range.
The high photostability of this FRET peptide provides a useful imaging probe. Many labs have used this kit for the high throughput screening of MMP inhibitors as potential anticancer drug candidates. This assay might be also used for monitoring cancer cells.
ab112147 should be stored Desiccated
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrix Metalloproteinases (MMPs) are a group of enzymes that break down extracellular matrix components. These are sometimes called matrixins. MMPs have a mass typically ranging from 19 to 120 kDa depending on the specific MMP enzyme. They are expressed in various tissues throughout the body including the skin lung liver and in the context of cancer cells. MMP activity is important for normal physiological processes like tissue remodeling and wound healing. Researchers frequently use MMP activity assay kits to measure the activity levels of these enzymes in biological samples for both research and clinical diagnostics.
Biological function summary
MMPs play significant roles in tissue remodeling and cell signaling. MMPs are not usually found as part of a complex but individual enzymes can be regulated through inhibitor proteins such as TIMPs (tissue inhibitors of metalloproteinases) which bind to MMPs and modulate their enzymatic activity. This regulation is essential to maintain tissue homeostasis and any imbalance may result in pathological conditions. MMPs influence processes such as morphogenesis angiogenesis and metastasis highlighting their importance in developmental biology and cancer research.
Pathways
MMPs are integral to processes involving the breakdown of the extracellular matrix. Notably MMPs are central components of the degradative pathways facilitating cell migration during immune responses and metastasis. MMPs interact closely with protein inhibitors like TIMPs and these interactions play key roles in the balance of proteolytic activity. Additionally MMPs relate to other proteins like integrins and cytokines linking them to pathways such as the TGF-beta signaling pathway and the Wnt signaling pathway.
MMPs have been implicated in conditions such as cancer and arthritis. In cancer abnormal MMP activity can elevate tumor invasion and metastasis where MMPs collaborate with proteins like E-cadherin and VEGF modulating the tumor microenvironment. In arthritis MMPs contribute to the degradation of cartilage intensifying inflammatory responses and tissue damage. Understanding MMP function and its inhibition offers therapeutic potential for managing these diseases.
