Description
Key facts
Assay time:1h,
Sample types:Tissue, Suspension cells, Adherent cells
Product details:
Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.
The amount of starting material can be as low as 10
cells or 1 mg of tissue. For best results, the cell number should be >10
cells or the tissue amount should be >10 mg.
A total of 100 standard extractions (using 10
cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 10
cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.
Histone purification is most often performed before the analysis of the global levels within a sample of post-translational histone modifications in western blot.
Chromatin immunoprecipitation (ChIP) is the most common technique used for the analysis of histone modifications at specific sites within the genome. Use a chromatin extraction kit instead of a histone purification kit for the preparation of samples for ChIP.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols
