Description
Key facts
Target:5(6)-Carboxyfluorescein diacetate succinimidyl esterSee target data,
Form:LiquidSee storage information,
Storage buffer:Constituents: 99% Dimethylsulfoxide, 0.56% 5(6)-Carboxyfluorescein diacetate N-hydroxysuccinimide ester
Product details:
CFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting and well retained within labeled cells. The provided CFSE is sufficient for ~1000 assays.
CFDA-SE (carboxyfluorescein diacetate succinimidyl ester) is a cell permeant molecule and is cleaved by intracellular esterases. The resulting fluorescent molecule CFSE (Carboxyfluorescein succinimidyl ester) indiscriminately with intracellular free amines to generate covalent fluorescent dye-protein conjugates. The result is live cells with an intracellular fluorescent label. At appropriate concentrations CFSE is non-toxic to cells and the fluorescence is retained after formaldehyde and alcohol fixation. CFSE labeled cells can be detected with any instrument or filter set compatible with FITC detection: Excitation(max)=492nm, Emission(max)=517nm.
Cell proliferation assays are indispensable for advancing scientific discovery and clinical applications.
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
