Description
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P05186,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alkaline Phosphatase Tissue Non-Specific also referred to as ALPL or alkaline phosphatase protein functions mechanically as a hydrolase enzyme. It catalyzes the dephosphorylation of molecules contributing to phosphate and mineral metabolism. This enzyme with a molecular mass of approximately 57 kDa exhibits expression in various tissues including liver bone and kidneys. Researchers can use techniques like alkaline phosphatase ELISA and immunohistochemistry to study its expression and localization.
Biological function summary
Alkaline phosphatase in tissues plays a pivotal role in bone mineralization and development. It functions to hydrolyze phosphate groups releasing inorganic phosphate necessary for bone and teeth formation. It forms part of a larger enzyme complex that interacts with extracellular substrates ensuring the continuous supply of phosphate ions. This enzyme in particular impacts cellular processes involving calcification and cellular differentiation.
Pathways
Alkaline phosphatase operates within phosphate metabolism and signaling pathways. Within the skeletal system it forms part of the regulatory mechanism for osteoblast activity and bone formation. It relates to proteins like osteopontin and bone sialoprotein which regulate the mineralization process. These pathways ensure the balance between phosphate ions and mineral deposition in bone tissue.
Alkaline phosphatase abnormalities correlate with hypophosphatasia and rickets. Hypophosphatasia results from mutations in the ALPL gene causing defective bone mineralization. In cases of rickets improper phosphate metabolism can occur due to dysfunctional enzyme activity. The imbalance in alkaline phosphatase activity also connects to disorders like hyperparathyroidism where calcium and phosphate homeostasis is disrupted involving proteins such as parathyroid hormone.
