Description
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P13489,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris-HCl buffer, 0.3% Glutathione
Product details:
This product was previously labelled as Ribonuclease Inhibitor.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ribonuclease Inhibitor (RI) also known as RAI is a protein weighing approximately 50 kDa and is responsible for binding to ribonucleases (RNases) to inhibit their activity. This protein is expressed in a variety of tissues but appears most abundantly in the cytoplasm of mammalian cells. RI is characterized by its leucine-rich repeat (LRR) structure which facilitates its high-affinity binding to target RNases effectively serving to regulate ribonuclease activity within cells.
Biological function summary
Ribonuclease Inhibitor participates in protecting cellular RNA from degradation by forming stable complexes with RNases. This interaction is important for maintaining RNA stability particularly within cellular environments where mRNA turnover must be tightly controlled. RI does not only inhibit free RNases but also regulates those RNases that are secreted or released subsequently. It does not have enzymatic activity itself serving more as a guard that ensures RNA integrity for processes such as transcription translation and overall gene expression.
Pathways
The function of Ribonuclease Inhibitor fits strategically into RNA metabolism and the regulation of its turnover under various physiological conditions. RI acts within the mRNA biogenesis and degradation pathways coordinating with other proteins involved in RNA processing such as Dicer. RI's role is essential in fine-tuning RNA levels and preventing unwanted cellulalr RNA cleavage which can be directed by pathways involving diverse sets of RNases across different cellular contexts.
Researchers have observed connections between Ribonuclease Inhibitor and conditions such as cancer and neurodegenerative diseases. For instance disrupting RI balance can result in excessive RNA degradation contributing to pathologies associated with abnormal mRNA destruction in cells. It often aligns in regulatory functions with proteins like angiogenin where disrupted RI-angiogenin interactions have been implicated in tumor growth and progression. Additionally RI's dysfunction is associated with neuroinflammatory conditions where RNA stability plays critical roles.
