Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Reacts with:Mouse, Human,
Sensitivity:= 2 ng,
Range:2 - 100 ng,
Assay time:2h 30m,
Assay Platform:Microplate reader
Product details:
Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
