Description
Key facts
Detection method:Fluorescent,
Assay Platform:Flow cytometer, Fluorescence microscope
Product details:
Live Dead Assay Kit ab115347 differentially labels live and dead cells with fluorescent dyes with a one-step live dead assay protocol. It is used for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy.
The Live Dead assay staining solution is a mixture of two fluorescent dyes that differentially label live and dead cells.
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm (similar to FITC).
The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm.
The Live Dead assay protocol uses a one-step staining procedure that is simple and fast. It can be used directly in cell culture media.
This assay is not suitable for use with fixed cells / cell fixation. The Live Dead assay staining solution provided is sufficient for ~1000 assays. **Related assays** Review the to learn about kits to perform a , and .
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
