Description
Key facts
Detection method:Fluorescent,
Sample types:Test Ligands,
Assay type:Quantitative,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
How the assay works
In Neutrophil Elastase Inhibitor Assay Kit protocol, Neutrophil Elastase hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent neutrophil elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
Neutrophil Elastase Inhibitor Assay Kit protocol summary
- Test inhibitor preparation Neutrophil elastase preparation
- Incubate for 5 mins at 37°C
- Add substrate mix
- Measure fluorescence (Ex/Em = 400/505 nm) in a kinetic mode
- Incubate for 30 mins at 37°C protected from light
- Measure fluorescence (Ex/Em = 400/505 nm) in a kinetic mode
Neutrophil Elastase Inhibitor Assay Kit has been used in a variety of sample type including:
C. mas L. extracts
B. officinalis herb extracts
Psidium guajava L. extracts
References:
1-Zagуrska-Dziok et al. 2023
2-Michalak M al. 2023
3-Bao S et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K782 Neutrophil Elastase Inhibitor Screening Kit (Fluorometric). K782-100 is the same size as the 100 test size of ab118971.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.
Biological function summary
The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.
Pathways
This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.
Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.
