Description
Key facts
Purity:>95% SDS-PAGE,
Expression system:Native,
Tags:Tag free,
Applications:SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P04133,
Animal free:No,
Carrier free:No,
Species:Human adenovirus 5,
Storage buffer:pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 2.19% Sodium chloride, 0.28% Bis-Tris propane
Product details:
Hexon protein naturally self assembles as part of the virus life cycle. When the protein is purified we also see evidence of self assembly such as rapid loss of protein upon serial dilution. The storage buffer and ELISA coating buffer play an important role in the likelihood of self assembly and thereby can affect results when using purified hexon as antigen in ELISAs and other such assays. Many common ELISA coating buffers increase self assembly in hexon protein as they are designed to increase efficiency of antigen adsorption. This results in a very high signal with the initial dilution but then the sensitivity tails off very rapidly. Diluting and coating the hexon in water alone slightly increases sensitivity but reduces strength; coating and diluting in the presence of a small amount of stabilising protein (such as BSA) seems to increase sensitivity for antigen coating significantly (although too much BSA can block high concentrations of hexon).
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Adenovirus Type 5 hexon also known as hexon protein or 5 protein is a major structural component of adenovirus capsid with a mass of approximately 960 kDa. Hexon proteins form the outer shell of the virus giving it stability and shape. These proteins are heavily expressed on the surface of human adenovirus serotype 5 a common virus strain used in therapeutic vector development. Hexon comprises three regions: the hypervariable regions conserved regions and the base that interacts with other capsid proteins.
Biological function summary
Adenovirus hexon proteins participate in the assembly and stability of the virus capsid complex. The hexon proteins interact with other components like penton base and fiber proteins to form a robust and protective viral coat. Their function involves binding to cell surface receptors such as the coxsackievirus and adenovirus receptor (CAR) allowing the virus to attach to and enter host cells. This interaction is key in viral infections and also in viral vector efficiency.
Pathways
The adenovirus Type 5 hexon protein plays important roles in immune evasion and viral entry pathways. The hexon protein helps the virus escape host immune detection by eluding neutralizing antibodies. The internal pathways also involve interactions with proteins like E1A which modulates host cell cycle pathways for viral replication. These interactions integrate hexon proteins into the broader adenoviral life cycle and infection processes.
Infections involving adenovirus Type 5 relate to respiratory illnesses and conjunctivitis. Respiratory infections often occur in crowded environments such as schools and military barracks and the hexon protein's interaction with the immune system can exacerbate symptoms. Additionally due to its prevalent role in viral vector design research on hexon relates to gene therapy contexts scrutinizing how hexon modifications impact disease treatment and host immune response. Hemagglutinin might connect to respiratory disorders linked to adenovirus making it also relevant in developing therapies.
