Description
Key facts
Detection method:Fluorescent,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:= 0.1 nmol/well,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Inosine Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.
Other Notes
This product was previously called K712 Inosine Fluorometric Assay Kit and Inosine Quantification Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Inosine a nucleoside plays a mechanical role in transfer RNA (tRNA) modification and purine metabolism. It commonly gets referred to as hypoxanthine riboside. Inosine has a molecular weight of 268.24 g/mol making it a relatively small molecule. It is broadly expressed in various tissues including muscle and nerve tissues due to its involvement in nucleotide synthesis and cellular energy metabolism.
Biological function summary
Inosine participates in diverse cellular processes beyond its role in nucleoside modification. Inosine forms part of a complex network where it impacts RNA editing and stability influencing protein translation. Its conversion to hypoxanthine via deamination is significant for the purine salvage pathway allowing cells to recycle purines efficiently. This activity underlines the importance of inosine in maintaining nucleotide balance and supporting cellular functions particularly in rapidly proliferating or energy-demanding cells.
Pathways
Inosine's involvement in the purine salvage and nucleotide metabolism pathways highlights its pivotal role in cellular homeostasis. The purine salvage pathway is essential for the recycling of nucleotides which inosine facilitates by converting to hypoxanthine and then to xanthine. Inosine interacts with proteins like xanthine oxidase part of the same metabolic pathway thereby ensuring efficient nucleotide utilization and preventing wasteful de novo synthesis.
Some studies have linked inosine to neurological disorders like multiple sclerosis and amyotrophic lateral sclerosis. Inosine shows potential in neuroprotection by increasing adenosine levels and promoting axonal regeneration. It interacts with adenosine deaminase which influences immune responses. Furthermore imbalances in inosine metabolism can exacerbate conditions related to oxidative stress and energy deficiency suggesting a therapeutic angle for inosine in specific neurological diseases.
