Description
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Mouse, Rat, Human,
Assay type:Semi-quantitative,
Assay time:5h,
Assay Platform:Microplate
Product details:
ab126433 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Akt protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Akt (Ser473) and total Akt in human, mouse and rat cell lysates (help normalize the results of phospho-Akt from different cell lysate being compared). A pan Akt antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Akt present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Akt (Ser473) or anti-pan-Akt is used to detect phosphorylated or total Akt. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Akt (Ser473) or total Akt bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The AKT family consists of three serine/threonine kinases: AKT1 AKT2 and AKT3 sometimes called protein kinase B (PKB). These kinases play a central role in various cellular processes. They have a mass of approximately 56-60 kDa and are expressed in numerous tissues including skeletal muscle pancreas and brain. AKT functions as an important regulator of cellular metabolism growth proliferation and survival. In their active form AKT proteins get phosphorylated; common phosphorylation targets include AKT ser473 (AKT ps473) which reflects their activation state.
Biological function summary
AKT kinases participate in multiple signaling cascades that control cell survival and growth. These proteins act downstream of receptor tyrosine kinases and are often associated with lipid kinase PI3K in signal transduction processes. The full activation of AKT kinases requires phosphorylation; p-AKT serves as a marker for activated AKT. The biological processes mediated by AKT include glycogen synthesis and glucose uptake influencing overall metabolic homeostasis.
Pathways
AKT kinases operate within critical pathways such as the PI3K/AKT/mTOR pathway and the insulin signaling pathway. These pathways regulate cellular metabolism proliferation and survival. AKT interacts with other proteins like mTOR and GSK3β integrating signals from growth factors and other external stimuli. By doing so AKT ensures proper cellular responses to external changes modulating processes like cell cycle entry.
AKT dysregulation associates with conditions like cancer and type 2 diabetes. In cancers AKT can become hyperactivated leading to unchecked cell growth and survival while in diabetes AKT function affects insulin signaling and glucose metabolism. AKT consequently interacts with proteins such as FOXO and BAD within these disease contexts influencing cell fate decisions and metabolic control. The AKT ELISA kit can be used to measure phosphorylated AKT levels offering insight into disease progression and treatment efficacy.
